Thin-layer chromatography silica gel plate - making What are the purpose of the thin-layer chromatography silica gel plate

by:Keyuan     2020-12-05
How to create a thin-layer chromatography silica gel plate, what are the purposes of thin-layer chromatography silica gel plate, want to know more about on thin layer chromatography silica gel plate, silica gel 10 years the old factory with you to understand it. How to create a thin-layer chromatography silica gel plate to silicone rubber plate activation in 100 ℃ oven for 30 min, cooled and draw a straight line from the bottom 2 cm, with a pencil mark spots position gently, of the distance between two points is 1? 1. 5cm。 b。 The quantity of samples, each sample is commonly 0. 8μL( As the case may be) , samples should be as small as possible, if you need to add, is the second time, third time drying the sample points at the same point. Each sample should be dry after the last point, in order to avoid the sample point excessive proliferation, thus affecting the result of the experiment; At room temperature after drying, quickly put sample wafers in advance to join expander in the chromatographic column, sealed chromatographic column, began to layer; d。 From the silicon rubber plate edge 2 cm distance, remove silicone plate edge from the silica gel plate, after the dry air at room temperature, 5 min at 50 ℃ dry, secondary cooling and then into a chromatographic column. At room temperature, dry, after the completion of coating development, choose the appropriate method of color to color of paint, and calculate the RF value. Chromogenic method: thin layer chromatography (TLC) method has a variety of color, the commonly used methods include uv ZhaoSheFa, fluorescent reagent method and the method of ultraviolet radiation, but should be different according to the material being tested to choose the appropriate color method. The above is the production and operation method of thin layer of silicon sheet is for reference only, : in operation or points should be paid attention to during the test. 1. Dissolve sample: the sample in similar polarity, high volatile organic solvents. 2. Sampling: control the sampling volume, from the bottom of the baseline for 2. 0 cm, diameter of sample points of control ( 5毫米) And the distance between each point ( 1 - 1. 5cm) , avoid the influence of diffusion ( The figure below) , when the sample can not damage the surface of the thin layer. 3. Layer: displays containers must be sealed and must add solvent in advance, to balance the solvent vapors, developer can not higher than that of the sample point, otherwise you won't get the RF value, unable to accurately determine the relative position of each component in the laminate. 4. Development agent selection criteria for: good solubility, separation of group separation of components of RF value at zero. 2 to 0. Between 8 and not react with measured components or adsorbent, the boiling point is moderate. Fruity and enrichment of low viscosity, a component, mixed solvent formula is best. 5. Edge effect, edge effect refers to the ct scan, the spots on either side of the plate moving faster than the middle point, and laterally offset. Reason is mixed solvent expands, weak polarity and low boiling point solvent volatilization on both sides of sheet, the sheet on the different proportion of developer, edge effect is produced. The most effective way to solve this kind of edge effect is cut into the two sheet at the end of the expander Angle. What are the USES thin-layer chromatography silica gel plate thin layer chromatography ( TLC) Silica gel plate has been widely used in medicine, pesticides, Chinese herbs, organic chemical products, food, and in the identification of trace impurities and of the main ingredients in the food, at present has been widely used in new materials for POM POM rod, also played an important role in judging cooking oil. Thin-layer chromatography silica gel plate type silicon rubber plate coating thickness can be divided into: analysis board: 0. Preparation of 2 mm plate: 0. 3 - 0. 4毫米,0。 4 - 0. 6毫米,0。 6 - 1 mm according to specifications, can be divided into: the type G H GF254HF254 divided into ordinary plate and efficient board! H is the abbreviation of Hard. G is the abbreviation of glutinous rice. F is the abbreviation of fluorescent lamp. Silica gel H: excluding adhesive, do not contain fluorescent agent; Silica gel G: do not contain fluorescent agent, including calcined gypsum adhesive; Silicone HF 254: containing fluorescent substances, can be used to observe 254 nm uv light fluorescence. Silica gel GF 254: containing calcined gypsum and contain fluorescent agent, can be used in the fluorescent observation of 254 nm uv light. GB/T1497- 1988 thin layer silica gel plate application of thin layer chromatography ( TLC) Silica gel plate has been widely used in medicine, pesticides, Chinese herbs, organic chemical products, food, and in the identification of trace impurities and of the main ingredients in the food, at present has been widely used in new materials for POM POM rod, also played an important role in judging cooking oil. Above is the rubber products co. , LTD. , provide relevant thin-layer chromatography silica gel plate, thin layer chromatography silica gel plate, what are the purposes of the relevant contents of hope to be of help. Recommend related products silicone watch silica gel hand ring silica gel bib pocket silicone ice silicone accessories food grade silica gel
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